Histochemical Localization of Intestinal Disaccharidases: Application to Peroral Biopsy Specimens

@article{Jos1967HistochemicalLO,
  title={Histochemical Localization of Intestinal Disaccharidases: Application to Peroral Biopsy Specimens},
  author={J. Jos and Jean Fr{\'e}zal and J. Rey and Martine Lamy},
  journal={Nature},
  year={1967},
  volume={213},
  pages={516-518}
}
THIS communication describes a technique for localizing intestinal disaccharidases developed from that of Dahlqvist and Brun1. Intestinal biopsies were obtained by a multipurpose suction biopsy tube2 from four normal children and five patients with sucrose and isomaltose “intolerance”. Intestinal specimens from rats and guinea-pigs have also been investigated by the same procedure. The biopsies and animal tissues were rapidly frozen and stored at −25°. Sections from SJJL were cut in a cryostat… CONTINUE READING