Histidyl-tRNA Synthetase Urzymes

  title={Histidyl-tRNA Synthetase Urzymes},
  author={Li Ken Li and Violetta Weinreb and Christopher Francklyn and Charles W. Carter},
  booktitle={The Journal of biological chemistry},
Four minimal (119-145 residue) active site fragments of Escherichia coli Class II histidyl-tRNA synthetase were constructed, expressed as maltose-binding protein fusions, and assayed for histidine activation as fusion proteins and after TEV cleavage, using the (32)PP(i) exchange assay. All contain conserved Motifs 1 and 2. Two contain an N-terminal… CONTINUE READING