Histidine-aromatic interactions in barnase. Elevation of histidine pKa and contribution to protein stability.

@article{Loewenthal1992HistidinearomaticII,
  title={Histidine-aromatic interactions in barnase. Elevation of histidine pKa and contribution to protein stability.},
  author={R E Loewenthal and Javier Sancho and A. R. Fersht},
  journal={Journal of molecular biology},
  year={1992},
  volume={224 3},
  pages={759-70}
}
Aromatic side-chains are found in the vicinity of histidine residues in many proteins and protein complexes. We have studied the interaction between a histidine residue (His18) and aromatic residues at position 94 in barnase. Three different techniques have been applied to show that Trp94 interacts more strongly with the protonated form of His18. The… CONTINUE READING