Histaminergic pharmacology of homo-oligomeric β3 γ-aminobutyric acid type A receptors characterized by surface plasmon resonance biosensor technology.

@article{Seeger2012HistaminergicPO,
  title={Histaminergic pharmacology of homo-oligomeric β3 γ-aminobutyric acid type A receptors characterized by surface plasmon resonance biosensor technology.},
  author={Christian Seeger and Tony Christopeit and Karoline Fuchs and Katharina Grote and Werner Sieghart and U Helena Danielson},
  journal={Biochemical pharmacology},
  year={2012},
  volume={84 3},
  pages={341-51}
}
A surface plasmon resonance biosensor assay was established for studying the interactions of 51 histaminergic and 15 GABAergic ligands with homo-oligomeric β3 GABA(A) receptors. Detergent solubilized receptors were successfully immobilized via affinity-capture on biosensor surfaces. The interaction kinetics of both histaminergic and GABAergic ligands were very rapid but affinities could be determined by steady-state analysis. Binding of several GABAergic ligands was observed, in agreement with… CONTINUE READING