Histamine measurement in whole blood and cell supernatants. A comparative study of two different radioimmunoassays.

@article{Rotz1991HistamineMI,
  title={Histamine measurement in whole blood and cell supernatants. A comparative study of two different radioimmunoassays.},
  author={Benjamin T. Rotz and Ayhan Savaşer and I Werthmann and S O Lau and Ulrich Wahn},
  journal={Allergy},
  year={1991},
  volume={46 7},
  pages={529-33}
}
Histamine as an important mediator of the allergic reaction has been measured by a number of methods both in whole blood and cell supernatants after in vitro release. We compared the fluorometric histamine assay with two radioimmunoassays and studied detection limit, recovery, cross-reactivity and accuracy in buffer-based standards as well as the sensitivity after IgE-mediated in vitro release from washed cells and whole blood. Our data indicate that the double-antibody RIA (Pharmacia) can be… CONTINUE READING