Histamine in tissue: determination by high-performance liquid chromatography after condensation with o-phthaldialdehyde.

@article{Skofitsch1981HistamineIT,
  title={Histamine in tissue: determination by high-performance liquid chromatography after condensation with o-phthaldialdehyde.},
  author={Gerhard Skofitsch and Alois Saria and Peter Holzer and Fred Lembeck},
  journal={Journal of chromatography},
  year={1981},
  volume={226 1},
  pages={53-9}
}
Histamine was determined by reversed-phase high-performance liquid chromatography in perchloric acid extracts after condensation with o-phthaldialdehyde. Fluorescence was monitored at 360 nm excitation and 450 nm emission wavelengths after elution with mixtures of 0.1 N acetic acid containing 0.1% pentanesulfonic acid and acetonitrile. The detection limit was 0.9 pmol of histamine. The histamine content was determined in rat whole brain, medulla oblongata, dorsal and ventral spinal cord, dorsal… CONTINUE READING

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