Histamine formed in stimulated human platelets is cytoplasmic.

  title={Histamine formed in stimulated human platelets is cytoplasmic.},
  author={Smita Saxena and A Mcnicol and Lorne J. Brandes and Aaron B Becker and Jonathan M. Gerrard},
  journal={Biochemical and biophysical research communications},
  volume={164 1},
The localization of histamine formed by human platelets in response to agonists was evaluated. 87 +/- 5% of the histamine in a suspension of platelets exposed to phorbol-12-myristate-13-acetate (PMA) was associated with the platelet pellet. Incubation of saponin-permeabilized platelets with the intracellular histamine antagonist, N,N-diethyl-2-[4-(phenylmethyl)phenoxy]ethanamine.HCl (DPPE), released 75 +/- 3.9% of the histamine into the supernatant. Under conditions where 90% of platelet… CONTINUE READING


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