Histamine dilutions modulate basophil activation

@article{Belon2003HistamineDM,
  title={Histamine dilutions modulate basophil activation},
  author={Philippe Belon and Jean Cumps and Madeleine Ennis and Pier Francesco Mannaioni and Marcel B. Roberfroid and J. L. Sainte-Laudy and Fred A. C. Wiegant},
  journal={Inflammation Research},
  year={2003},
  volume={53},
  pages={181-188}
}
AbstractBackground:In order to demonstrate that high dilutions of histamine are able to inhibit basophil activation in a reproducible fashion, several techniques were used in different research laboratories. Objective:The aim of the study was to investigate the action of histamine dilutions on basophil activation. Methods:Basophil activation was assessed by alcian blue staining, measurement of histamine release and CD63 expression. Study 1 used a blinded multi-centre approach in 4 centres… Expand
Replication study concerning the effects of homeopathic dilutions of histamine on human basophil degranulation in vitro.
TLDR
Histamine at the dilutions 10(-2)M and 10(-22)M was associated with a significant inhibition of basophil degranulation, but the previously reported large effects of homeopathic histamine dilutions on basophils function of the examined donor were not confirmed. Expand
Inhibition of basophil activation by histamine: a sensitive and reproducible model for the study of the biological activity of high dilutions.
TLDR
Successively, using different models to study of human and murine basophil activation, it is demonstrated that high dilutions of histamine, in the range of 15-17CH induce a reproducible biological effect. Expand
Basophil models of homeopathy: a sceptical view.
  • M. Ennis
  • Medicine
  • Homeopathy : the journal of the Faculty of Homeopathy
  • 2010
TLDR
It is recommended that a multi-centre trial be performed to hopefully put an end to this "never-ending story" of trying to find out if high dilutions of histamine have a negative feedback effect on the activation of basophils by anti-IgE. Expand
Improvement of flow cytometric analysis of basophil activation inhibition by high histamine dilutions. A novel basophil specific marker: CD 203c.
TLDR
These new flow cytometric protocols confirmed that high dilutions of histamine may inhibit basophil activation and that the inhibitory effect is not restricted to IgE-dependent activation. Expand
Inhibition of CD203c membrane up-regulation in human basophils by high dilutions of histamine: a controlled replication study
TLDR
It is shown that low and high dilutions of histamine inhibit CD203c up-regulation in anti-IgE stimulated basophils, using a strictly standardized flow cytometry protocol and a new dilution/succussion procedure. Expand
Differential effect of storage on molecular and ultra-molecular dilutions of histamine
TLDR
The effect of storage on the activity of these histamine dilutions is presented, which led to the confirmation that high dilutions of histamine were capable of inhibiting anti-IgE or fMLP induced basophil activation. Expand
Immunological models in high dilution research following M Bastide.
TLDR
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Survey of immuno-allergological ultra high dilution research.
  • B. Poitevin
  • Medicine
  • Homeopathy : the journal of the Faculty of Homeopathy
  • 2015
TLDR
Despite considerable research activity in immuno-allergology and a great increase in the number of publications, there is still not in this domain a "gold standard" trial in basic research in homeopathy. Expand
Confirmation of biological effects of high dilutions. Effects of submolecular concentrations of histamine and 1-, 3- and 4-methylhistamines on human basophil activation
TLDR
The IgE/CD203c cytometric protocol is used to compare the activity of histamine and 1-, 3-, and 4-methylhistamines on basophils selected by anti-IgE (FITC) and cell activation measured by CD203c upregulation and the artefact hypothesis is tested. Expand
Basophils, basophilia and helminth infections.
TLDR
Basic basophil biology and physiology is reviewed, the evidence for the presence of basophilia in helminth infections is evaluated, and the possible roles basophils serve in the immune response to helmineth infections are focused on. Expand
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