Histamine dilutions modulate basophil activation

@article{Belon2003HistamineDM,
  title={Histamine dilutions modulate basophil activation},
  author={Philippe Belon and Jean Cumps and Madeleine Ennis and Pier Francesco Mannaioni and Marcel B. Roberfroid and J. L. Sainte-Laudy and Fred A. C. Wiegant},
  journal={Inflammation Research},
  year={2003},
  volume={53},
  pages={181-188}
}
AbstractBackground:In order to demonstrate that high dilutions of histamine are able to inhibit basophil activation in a reproducible fashion, several techniques were used in different research laboratories. Objective:The aim of the study was to investigate the action of histamine dilutions on basophil activation. Methods:Basophil activation was assessed by alcian blue staining, measurement of histamine release and CD63 expression. Study 1 used a blinded multi-centre approach in 4 centres… 

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...

References

SHOWING 1-10 OF 38 REFERENCES

Flow-cytometric analysis of basophil activation: inhibition by histamine at conventional and homeopathic concentrations

The aim of this study was to confirm that a 2-colour (anti-IgE FITC and anti-CD63 R-PE) flow cytometric method can quantify basophil activation and to investigate the effects of histamine on basophils activation.

Application of flow cytometry to the analysis of the immunosuppressive effect of histamine dilutions on human basophil activation: Effect of cimetidine

The aim of this study was to reinvestigate the ability of cimetidine to reverse the immunomodulatory effects of histamine using the flow cytometric method and confirm the biological activity of ultra low doses of histamines.

Inhibition of human basophil activation by high dilutions of histamine

The inhibition of anti-IgE induced human basophil activation by histamine dilutions ranging from 10−10M to 10−38M, the most frequent inhibition observed being for 10−18, 10−20, and 10−32M, in agreement with the previous results.

Analysis of anti-IgE and allergen induced human basophil activation by flow cytometry. Comparison with histamine release

Flow cytometry, as a popular method often used in the immunology and haematology departments of clinical laboratories, may represent a new alternative for allergy diagnosis and basophil pharmacology.

The effects of the H1 and H2 antihistamines on "allergic" histamine release and its inhibition by histamine.

The anti-anaphylactic effects of the H1 antagonists probably play no therapeutic role but it is suggested that drugs structurally similar to the phenothiazine antihistamines should be developed for clinical testing.

Mechanical agitation of very dilute antiserum against IgE has no effect on basophil staining properties

The effect of extreme dilutions of anti-IgE, reported by Davenas et al.2, needs further clarification and that in this process the reproducibility of results between experimenters should be carefully determined.

Human basophil degranulation triggered by very dilute antiserum against IgE

When human polymorphonuclear basophils are exposed to anti-IgE antibodies, they release histamine from their intracellular granules and change their staining properties, and transmission of the biological information could be related to the molecular organization of water.

Evidence for H 2-receptor-mediated inhibition of histamine release from isolated rat mast cells

H-receptor agonists and antagonists inhibited the release of histamine from isolated purified rat mast cells evoked by compound 48/80 and acetylcholine, demonstrating the existence of a histamine feed-back inhibition of immediate hypersensitivity reactions mediated by H2-receptors.

Human basophil degranulation is not triggered by very dilute antiserum against human IgE

The findings of Benveniste and co-workers, who reported in 1988 that degranulation of human basophil leukocytes is triggered by very dilute antiserum against IgE, are reproduced.