Histamine dilutions modulate basophil activation

  title={Histamine dilutions modulate basophil activation},
  author={Philippe Belon and Jean Cumps and Madeleine Ennis and Pier Francesco Mannaioni and Marcel B. Roberfroid and J. L. Sainte-Laudy and Fred A. C. Wiegant},
  journal={Inflammation Research},
AbstractBackground:In order to demonstrate that high dilutions of histamine are able to inhibit basophil activation in a reproducible fashion, several techniques were used in different research laboratories. Objective:The aim of the study was to investigate the action of histamine dilutions on basophil activation. Methods:Basophil activation was assessed by alcian blue staining, measurement of histamine release and CD63 expression. Study 1 used a blinded multi-centre approach in 4 centres… 

Basophil models of homeopathy: a sceptical view.

  • M. Ennis
  • Biology
    Homeopathy : the journal of the Faculty of Homeopathy
  • 2010

Inhibition of CD203c membrane up-regulation in human basophils by high dilutions of histamine: a controlled replication study

It is shown that low and high dilutions of histamine inhibit CD203c up-regulation in anti-IgE stimulated basophils, using a strictly standardized flow cytometry protocol and a new dilution/succussion procedure.

Differential effect of storage on molecular and ultra-molecular dilutions of histamine

The effect of storage on the activity of these histamine dilutions is presented, which led to the confirmation that high dilutions of histamine were capable of inhibiting anti-IgE or fMLP induced basophil activation.

Immunological models in high dilution research following M Bastide.

Survey of immuno-allergological ultra high dilution research.

  • B. Poitevin
  • Biology, Medicine
    Homeopathy : the journal of the Faculty of Homeopathy
  • 2015

Confirmation of biological effects of high dilutions. Effects of submolecular concentrations of histamine and 1-, 3- and 4-methylhistamines on human basophil activation

The IgE/CD203c cytometric protocol is used to compare the activity of histamine and 1-, 3-, and 4-methylhistamines on basophils selected by anti-IgE (FITC) and cell activation measured by CD203c upregulation and the artefact hypothesis is tested.

Basophils, basophilia and helminth infections.

Basic basophil biology and physiology is reviewed, the evidence for the presence of basophilia in helminth infections is evaluated, and the possible roles basophils serve in the immune response to helmineth infections are focused on.

IgE-mediated 15-hydroxyeicosatetraenoic acid (15-HETE) generation by peripheral blood leukocytes: its association with basophil activation

Allergen-induced, IgE-mediated activation of basophils is associated with a significant increase in 15-HETE generation, seen in patients with clinical symptoms of birch pollen allergy.



Flow-cytometric analysis of basophil activation: inhibition by histamine at conventional and homeopathic concentrations

The aim of this study was to confirm that a 2-colour (anti-IgE FITC and anti-CD63 R-PE) flow cytometric method can quantify basophil activation and to investigate the effects of histamine on basophils activation.

Application of flow cytometry to the analysis of the immunosuppressive effect of histamine dilutions on human basophil activation: Effect of cimetidine

The aim of this study was to reinvestigate the ability of cimetidine to reverse the immunomodulatory effects of histamine using the flow cytometric method and confirm the biological activity of ultra low doses of histamines.

Inhibition of human basophil activation by high dilutions of histamine

The inhibition of anti-IgE induced human basophil activation by histamine dilutions ranging from 10−10M to 10−38M, the most frequent inhibition observed being for 10−18, 10−20, and 10−32M, in agreement with the previous results.

Analysis of anti-IgE and allergen induced human basophil activation by flow cytometry. Comparison with histamine release

Flow cytometry, as a popular method often used in the immunology and haematology departments of clinical laboratories, may represent a new alternative for allergy diagnosis and basophil pharmacology.

The effects of the H1 and H2 antihistamines on "allergic" histamine release and its inhibition by histamine.

The anti-anaphylactic effects of the H1 antagonists probably play no therapeutic role but it is suggested that drugs structurally similar to the phenothiazine antihistamines should be developed for clinical testing.

Mechanical agitation of very dilute antiserum against IgE has no effect on basophil staining properties

The effect of extreme dilutions of anti-IgE, reported by Davenas et al.2, needs further clarification and that in this process the reproducibility of results between experimenters should be carefully determined.

Human basophil degranulation triggered by very dilute antiserum against IgE

When human polymorphonuclear basophils are exposed to anti-IgE antibodies, they release histamine from their intracellular granules and change their staining properties, and transmission of the biological information could be related to the molecular organization of water.

Evidence for H 2-receptor-mediated inhibition of histamine release from isolated rat mast cells

H-receptor agonists and antagonists inhibited the release of histamine from isolated purified rat mast cells evoked by compound 48/80 and acetylcholine, demonstrating the existence of a histamine feed-back inhibition of immediate hypersensitivity reactions mediated by H2-receptors.

Human basophil degranulation is not triggered by very dilute antiserum against human IgE

The findings of Benveniste and co-workers, who reported in 1988 that degranulation of human basophil leukocytes is triggered by very dilute antiserum against IgE, are reproduced.