Histamine H1 receptors and inositol phosphate formation in rat thalamus.

@article{SoriaJasso1997HistamineHR,
  title={Histamine H1 receptors and inositol phosphate formation in rat thalamus.},
  author={Luis Enrique Soria-Jasso and R Bahena-Trujillo and Jos{\'e} Antonio Arias-Monta{\~n}o},
  journal={Neuroscience letters},
  year={1997},
  volume={225 2},
  pages={117-20}
}
In membranes of rat thalamus the density of histamine H1 receptors, as estimated from saturation curves with [3H]mepyramine, was 66 +/- 5 fmol.mg protein-1 (Kd 1.3 +/- 0.1 nM). Specific [3H]mepyramine binding was inhibited by mepyramine (best fit to one-site model, Kd 2.2 +/- 0.2 nM) and by histamine (best fit to a two-site model, Ki high 0.35 +/- 0.04 microM and 54 +/- 7% of binding sites; Ki low 7.0 +/- 1.1 microM). In the presence of 300 microM GppNHp (5'-guanylylimidodiphosphate) the… CONTINUE READING