His-tag protein monitoring by a fast mix-and-measure immunoassay

  title={His-tag protein monitoring by a fast mix-and-measure immunoassay},
  author={Thomas Kreisig and Agneta A. Prasse and Kristin Zscharnack and Daniela Volke and Thole Zuchner},
  booktitle={Scientific reports},
Here, we present a fast mix-and-measure immunoassay for the specific semiquantitative detection of His-tagged proteins, for example in E. coli cell lysate. The assay is based on Förster resonance energy transfer (FRET) between a lanthanide dye-labeled low-affinity His-peptide and an acceptor-labeled anti-His-tag antibody. The targeted His-tag protein in the… CONTINUE READING