Hin-mediated site-specific recombination requires two 26 by recombination sites and a 60 by recombinational enhancer

@article{Johnson1985HinmediatedSR,
  title={Hin-mediated site-specific recombination requires two 26 by recombination sites and a 60 by recombinational enhancer},
  author={R. Johnson and M. Simon},
  journal={Cell},
  year={1985},
  volume={41},
  pages={781-791}
}
  • R. Johnson, M. Simon
  • Published 1985
  • Biology, Medicine
  • Cell
  • The alternate expression of flagellin genes in Salmonella is the result of an inversion of a 996 bp segment of chromosomal DNA. We have analyzed the components of this site-specific recombination reaction in an in vitro system derived from E. coli. Efficient Hin-mediated inversion requires the 20,000 MW Hin protein and a proteinase K-sensitive host component. The supercoiled DNA substrate must contain two 26 bp recombination sites in inverted configuration and a 60 bp sequence that increases… CONTINUE READING
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