Hin-mediated site-specific recombination requires two 26 by recombination sites and a 60 by recombinational enhancer

@article{Johnson1985HinmediatedSR,
  title={Hin-mediated site-specific recombination requires two 26 by recombination sites and a 60 by recombinational enhancer},
  author={R. Johnson and M. Simon},
  journal={Cell},
  year={1985},
  volume={41},
  pages={781-791}
}
  • R. Johnson, M. Simon
  • Published 1985
  • Biology, Medicine
  • Cell
    • The alternate expression of flagellin genes in Salmonella is the result of an inversion of a 996 bp segment of chromosomal DNA. We have analyzed the components of this site-specific recombination reaction in an in vitro system derived from E. coli. Efficient Hin-mediated inversion requires the 20,000 MW Hin protein and a proteinase K-sensitive host component. The supercoiled DNA substrate must contain two 26 bp recombination sites in inverted configuration and a 60 bp sequence that increases… CONTINUE READING
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        References

        SHOWING 1-10 OF 51 REFERENCES
        Role for DNA homology in site-specific recombination. The isolation and characterization of a site affinity mutant of coliphage lambda.
        • 90
        Studies on the properties of P1 site-specific recombination: Evidence for topologically unlinked products following recombination
        • 286
        Bacteriophage P1 site-specific recombination. Purification and properties of the Cre recombinase protein.
        • 281
        P1 site-specific recombination: nucleotide sequence of the recombining sites.
        • 292
        • PDF
        DNA inversions in the chromosome of Escherichia coli and in bacteriophage Mu: relationship to other site-specific recombination systems.
        • 83
        • PDF
        Gin‐mediated site‐specific recombination in bacteriophage Mu DNA: overproduction of the protein and inversion in vitro
        • 33
        Analysis of the gamma delta res site. Sites required for site-specific recombination and gene expression.
        • 6
        Site-specific relaxation and recombination by the Tn3 resolvase: Recognition of the DNA path between oriented res sites
        • 135
        • PDF
        Analysis of the γδ res site: Sites required for site-specific recombination and gene expression
        • 29
        Transposon-mediated site-specific recombination: A defined in vitro system
        • 171