Highly stable glycosylated serine protease from the medicinal plant Euphorbia milii.

@article{Yadav2006HighlySG,
  title={Highly stable glycosylated serine protease from the medicinal plant Euphorbia milii.},
  author={Subhash Yadav and Monu Pande and Medicherla Venkata Jagannadham},
  journal={Phytochemistry},
  year={2006},
  volume={67 14},
  pages={1414-26}
}
A serine protease, named as "Milin" was purified to homogeneity from the latex of Euphorbia milii, a medicinal plant of Euphorbiaceae family. The molecular mass (SDS-PAGE), optimum pH and temperature of the enzyme were 51kDa, pH 8.0 and 60 degrees C, respectively. Milin retains full proteolytic activity over a wide range of pH (5.5-12) and temperature (up to 65 degrees C) with casein and azoalbumin as substrates. The activity of milin is inhibited by serine proteases inhibitors like PMSF, APMSF… CONTINUE READING

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