[Highly sensitive enzyme-linked assay based on monoclonal antibodies for detection of Brucella antigens].

Abstract

Mice monoclonal antibodies against lypopolysaccharides (LPS) of Brucella abortus has been obtained and characterized. The antibodies detected LPS of B. abortus, B. melitensis and B. suis with high sensivity and specificity and did not react with LPS of Yersinia enterocolitica O:3, Y. enterocolitica O:9, Salmonella typhimurium, and Francisella tularensis. It has been shown that interaction of monoclonal antibodies and LPS of Brucella species can be critically dependent from buffer system. Obtained monoclonal antibodies allowed to develop highly sensitive assay which was able to detect antigens of Brucella species in concentrations 0.05 - 0.1 ng/ml. The assay can be used for detection and identification of Brucella species.

Cite this paper

@article{Feldsherova2007HighlySE, title={[Highly sensitive enzyme-linked assay based on monoclonal antibodies for detection of Brucella antigens].}, author={A A Fel'dsherova and D. A. {\'E}l'gort and M. V. Konopleva and Iu S Khats and O Iu Tret'iakov and Iu K Kulakov and T A Tolmacheva and Michael M Zheludkov and Anatoly P Suslov}, journal={Zhurnal mikrobiologii, epidemiologii, i immunobiologii}, year={2007}, volume={1}, pages={52-7} }