Highly sensitive determination of microRNA using target-primed and branched rolling-circle amplification.


One-nucleotide differences in microRNAs (miRNAs) can be discriminated in an assay based on a branched rolling-circle amplification (BRCA) reaction and fluorescence quantification. With the proposed method miRNA can be detected at concentrations as low as 10 fM, and the miRNA in a total RNA sample of a few nanograms can be determined. 
DOI: 10.1002/anie.200805665


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