Highly sensitive detection and quantification of the pathogen Yersinia ruckeri in fish tissues by using real-time PCR

@article{Bastardo2012HighlySD,
  title={Highly sensitive detection and quantification of the pathogen Yersinia ruckeri in fish tissues by using real-time PCR},
  author={Asmine Bastardo and Carmen Ravelo and Jes{\'u}s L Romalde},
  journal={Applied Microbiology and Biotechnology},
  year={2012},
  volume={96},
  pages={511-520}
}
Yersinia ruckeri is the causative agent of enteric redmouth diseases (ERM) and one of the major bacterial pathogens causing losses in salmonid aquaculture. Since recent ERM vaccine breakdowns have been described mostly attributed to emergence of Y. ruckeri biotype 2 strains, rapid, reproducible, and sensitive methods for detection are needed. In this study, a real-time polymerase chain reaction (PCR) primer/probe set based on recombination protein A (recA) gene was designed and optimized to… CONTINUE READING

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