Highly sensitive amplified electronic detection of DNA by biocatalyzed precipitation of an insoluble product onto electrodes.

@article{Patolsky2003HighlySA,
  title={Highly sensitive amplified electronic detection of DNA by biocatalyzed precipitation of an insoluble product onto electrodes.},
  author={Fernando Patolsky and Amir Lichtenstein and Itamar Willner},
  journal={Chemistry},
  year={2003},
  volume={9 5},
  pages={1137-45}
}
The amplified detection of a target DNA, based on the alkaline phosphatase oxidative hydrolysis of the soluble 5-bromo-4-chloro-3-indoyl phosphate to the insoluble indigo product as an amplification path, is addressed by two different sensing configurations. The accumulation of the insoluble product on Au electrodes or Au/quartz crystals alters the interfacial electron-transfer resistance at the Au electrode or the mass associated with the piezoelectric crystal, thus enabling the quantitative… CONTINUE READING
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