Highly sensitive PCR assay for routine diagnosis of African swine fever virus in clinical samples.

@article{Agero2003HighlySP,
  title={Highly sensitive PCR assay for routine diagnosis of African swine fever virus in clinical samples.},
  author={Montserrat Ag{\"u}ero and Jovita Fern{\'a}ndez and Luis Jos{\'e} Romero and C S{\'a}nchez Mascaraque and Marisa Arias and Jos{\'e} M. S{\'a}nchez-Vizca{\'i}no},
  journal={Journal of clinical microbiology},
  year={2003},
  volume={41 9},
  pages={4431-4}
}
This work provides a novel, highly sensitive, hot start PCR method for rapid and specific detection of African swine fever virus (ASFV) that can be used as a routine diagnostic test for ASFV in surveillance, control, and eradication programs. A confirmatory test of the specificity of this method based on restriction endonuclease analysis was also developed. 

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