Highly reproducible absolute quantification of Mycobacterium tuberculosis complex by digital PCR.

@article{Devonshire2015HighlyRA,
  title={Highly reproducible absolute quantification of Mycobacterium tuberculosis complex by digital PCR.},
  author={Alison S. Devonshire and Isobella Honeyborne and Alice Gutteridge and Alexandra S Whale and Gavin J. Nixon and Philip Wilson and Gerwyn M. Jones and Timothy D McHugh and Carole A. Foy and Jim F Huggett},
  journal={Analytical chemistry},
  year={2015},
  volume={87 7},
  pages={3706-13}
}
Digital PCR (dPCR) offers absolute quantification through the limiting dilution of template nucleic acid molecules and has the potential to offer high reproducibility. However, the robustness of dPCR has yet to be evaluated using complex genomes to compare different dPCR methods and platforms. We used DNA templates from the pathogen Mycobacterium… CONTINUE READING