Highly regionalized neuronal expression of monocyte chemoattractant protein‐1 (MCP‐1/CCL2) in rat brain: Evidence for its colocalization with neurotransmitters and neuropeptides

  title={Highly regionalized neuronal expression of monocyte chemoattractant protein‐1 (MCP‐1/CCL2) in rat brain: Evidence for its colocalization with neurotransmitters and neuropeptides},
  author={Ghazal Banisadr and Romain-Daniel Gosselin and Patricia M{\'e}chighel and Patrick Kitabgi and William Rost{\`e}ne and St{\'e}phane M{\'e}lik Parsadaniantz},
  journal={Journal of Comparative Neurology},
The monocyte chemoattractant protein‐1 (MCP‐1/CCL2) and its receptor CCR2 are key modulators of immune functions. In the nervous system, MCP‐1/CCL2 is implicated in neuroinflammatory pathologies. However, cerebral functions of MCP‐1/CCL2 under normal conditions are still unclear. In this study, using reverse transcriptase‐polymerase chain reaction (RT‐PCR) and specific rat MCP‐1 enzyme‐linked immunosorbent assay (ELISA) approaches, we observed that MCP‐1/CCL2 mRNA and protein were expressed in… 
Constitutive neuronal expression of CCR2 chemokine receptor and its colocalization with neurotransmitters in normal rat brain: Functional effect of MCP‐1/CCL2 on calcium mobilization in primary cultured neurons
The constitutive neuronal CCR2 expression in selective brain structures suggests that this receptor could be involved in neuronal communication and possibly associated with cholinergic and dopaminergic neurotransmission and related disorders.
CCR3, CCR2A and macrophage inflammatory protein (MIP)‐1α, monocyte chemotactic protein‐1 (MCP‐1) in the mouse hippocampus during and after pilocarpine‐induced status epilepticus (PISE)
The downregulation of CCR3, CCR2A, MIP‐1α and MCP‐1 in the hippocampal neurones at the acute stage during and after PISE may weaken the neuroprotective mechanisms.
Glial- and Neuronal-Specific Expression of CCL5 mRNA in the Rat Brain
The expression of CCL5 mRNA and protein, together with its receptors, in selected brain cell populations proposes that this chemokine could be involved in neuronal/glial communication.
Role of monocyte chemoattractant protein‐1 (MCP‐1/CCL2) in migration of neural progenitor cells toward glial tumors
An in vivo model for NPC migration is found and evidence of NPC migration toward areas of MCP‐1 infusion in rat brains is found, which may be used to enhance delivery of cytotoxic agents to brain tumor cells.
Critical roles of astrocytic-CCL2-dependent monocyte infiltration in a DJ-1 knockout mouse model of delayed brain repair.
It is shown that a DJ-1 deficiency attenuates monocyte infiltration into the damaged brain owing to a decrease in C-C motif chemokine ligand 2 (CCL2) expression in astrocytes, which led to delay in repair of brain injury.
It is suggested that MCP-1/CCR2 signaling plays an important role in ethanol-induced microglial activation/neuroinflammation and neurodegeneration in the developing brain and also plays anImportant role in developmental alcohol exposure induced long-lasting behavioral deficits in adolescence and adulthood.
CCL2/CCR2 system in neuroepithelial radial glia progenitor cells: involvement in stimulatory, sexually dimorphic effects of maternal ethanol on embryonic development of hypothalamic peptide neurons
Investigating in the embryo the developmental origins of this CCL2/CCR2-mediated stimulatory effect of maternal ethanol exposure on MCH neurons provides new evidence for how an inflammatory chemokine pathway functions within neuroprogenitor cells to mediate ethanol’s long-lasting, stimulatory effects on peptide neurons linked to adolescent drinking behavior.
TGFβRI antagonist inhibits HIV-1 Nef-induced CC chemokine family ligand 2 (CCL2) in the brain and prevents spatial learning impairment
The findings suggest that TGFβ-1 signaling is an intriguing target to reduce neuroHIV, and rats treated with Nef showed deficits in spatial learning and memory in the novel location recognition task.


Highly regionalized distribution of stromal cell‐derived factor‐1/CXCL12 in adult rat brain: constitutive expression in cholinergic, dopaminergic and vasopressinergic neurons
The first evidence that SDF‐1/CXCL12 is constitutively expressed in cholinergic neurons in the medial septum and substantia innominata and in dopaminergic neuron in substantia nigra pars compacta and the ventral tegmental area is provided.
Distribution, cellular localization and functional role of CCR2 chemokine receptors in adult rat brain
The results provide the first evidence for constitutive CCR2 receptor expression with precise neuroanatomical and cellular localizations in the brain, and its regulation during an inflammatory process, suggesting that MCP‐1/CCL2 and C CR2 play important physiological and pathophysiological role(s) in the CNS.
Regulation of the gene encoding the monocyte chemoattractant protein 1 (MCP‐1) in the mouse and rat brain in response to circulating LPS and proinflammatory cytokines
The genetic regulation and fine cellular distribution of the monocyte chemoattractant protein‐1 (MCP‐1) in the brain of mice and rats in response to systemic immune insults provide the anatomical evidence that MCP‐ 1 is expressed within specific populations of cells in responseto systemic inflammatory molecules that use NF‐κB as intracellular signaling system.
Acute Excitotoxic Injury Induces Expression of Monocyte Chemoattractant Protein-1 and Its Receptor, CCR2, in Neonatal Rat Brain
It is demonstrated that in the neonatal brain, acute excitotoxic injury stimulates expression of both MCP-1 and its receptor, CCR2, and suggests that M CP-1 regulates the microglial/monocyte response to acute brain injury.
Expression of monocyte chemoattractant protein-1 and macrophage inflammatory protein-1 after focal cerebral ischemia in the rat
Astrocyte expression of mRNA encoding cytokines IP‐10 and JE/MCP‐1 in experimental autoimmune encephalomyelitis
  • R. Ransohoff, T. Hamilton, V. Tuohy
  • Biology
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 1993
In situ hybridizations showed, unexpectedly, that astrocytes were the major source of mRNAs encoding IP‐10 and JE/MCP‐1, which implicateAstrocyte‐derived cytokines as potential chemoattractants for inflammatory cells during EAE.
Controlled recruitment of monocytes and macrophages to specific organs through transgenic expression of monocyte chemoattractant protein-1.
The mononuclear cell infiltrate in the brain can be significantly amplified by LPS treatment, suggesting that the recruitment properties of MCP-1 can be potentiated by additional factors.