Highly parallel direct RNA sequencing on an array of nanopores

  title={Highly parallel direct RNA sequencing on an array of nanopores},
  author={Daniel R. Garalde and Elizabeth A. Snell and Daniel Jachimowicz and Botond Sipos and J. H. Lloyd and Mark Bruce and Nadia Pantic and Tigist Admassu and Phillip James and Anthony Warland and Michael Jordan and Jonah Ciccone and Sabrina Serra and Jemma Keenan and Samuel A. M. Martin and Luke McNeill and Elizabeth Jayne Wallace and Lakmal Jayasinghe and Christopher V.E. Wright and Javier Blasco and Stephen G. Young and Denise Brocklebank and Sissel Juul and James Clarke and Andrew John Heron and Daniel J. Turner},
  journal={Nature Methods},
Sequencing the RNA in a biological sample can unlock a wealth of information, including the identity of bacteria and viruses, the nuances of alternative splicing or the transcriptional state of organisms. However, current methods have limitations due to short read lengths and reverse transcription or amplification biases. Here we demonstrate nanopore direct RNA-seq, a highly parallel, real-time, single-molecule method that circumvents reverse transcription or amplification steps. This method… 

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