Highly efficient modification of bacterial artificial chromosomes (BACs) using novel shuttle vectors containing the R6Kgamma origin of replication.

@article{Gong2002HighlyEM,
  title={Highly efficient modification of bacterial artificial chromosomes (BACs) using novel shuttle vectors containing the R6Kgamma origin of replication.},
  author={Shiaoching Gong and Xiangdong William Yang and Chenjian Li and Nathaniel Heintz},
  journal={Genome research},
  year={2002},
  volume={12 12},
  pages={1992-8}
}
Bacterial artificial chromosome (BAC) mediated transgenesis has proven to be a highly reliable way to obtain accurate transgene expression for in vivo studies of gene expression and function. A rate-limiting step in use of this technology to characterize large numbers of genes has been the process with which BACs can be modified by homologous recombination in Escherichia coli. We report here a highly efficient method for modifying BACs by using a novel set of shuttle vectors that contain the… CONTINUE READING
122 Citations
33 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 122 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 33 references

A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA

  • E. C. Lee, D. Yu, +5 authors N. G. Copeland
  • Genomics 73: 56–65.
  • 2001
Highly Influential
4 Excerpts

Similar Papers

Loading similar papers…