Highly efficient isothermal DNA amplification system using three elements of 5'-DNA-RNA-3' chimeric primers, RNaseH and strand-displacing DNA polymerase.

@article{Mukai2007HighlyEI,
  title={Highly efficient isothermal DNA amplification system using three elements of 5'-DNA-RNA-3' chimeric primers, RNaseH and strand-displacing DNA polymerase.},
  author={Hiroyuki Mukai and Takashi Uemori and Osamu Takeda and Eiji Kobayashi and Junko Yamamoto and Kazue Nishiwaki and Tatsuji Enoki and H. Sagawa and Kiyozo Asada and I. Kato},
  journal={Journal of biochemistry},
  year={2007},
  volume={142 2},
  pages={273-81}
}
We developed an efficient method of isothermally amplifying DNA termed ICAN, Isothermal and Chimeric primer-initiated Amplification of Nucleic acids. This method allows the amplification of target DNA under isothermal conditions at around 55 degrees C using only a pair of 5'-DNA-RNA-3' chimeric primers, a thermostable RNaseH and a DNA polymerase with strong strand-displacing activity. ICAN is capable of amplifying DNA at least several times greater than the amount produced with PCR by… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-9 of 9 extracted citations

Similar Papers

Loading similar papers…