Highly efficient in vitro site-specific recombination system based on streptomyces phage phiBT1 integrase.

@article{Zhang2008HighlyEI,
  title={Highly efficient in vitro site-specific recombination system based on streptomyces phage phiBT1 integrase.},
  author={Lin Zhang and Xijun Ou and Guo-Ping Zhao and Xiaoming Ding},
  journal={Journal of bacteriology},
  year={2008},
  volume={190 19},
  pages={6392-7}
}
The Streptomyces phage phiBT1 encodes a site-specific integrase of the large serine recombinase subfamily. In this report, the enzymatic activity of the phiBT1 integrase was characterized in vitro. We showed that this integrase has efficient integration activity with substrate DNAs containing attB and attP sites, independent of DNA supercoiling or cofactors. Both intra- and intermolecular recombinations proceed with rapid kinetics. The recombination is highly specific, and no reactions are… CONTINUE READING

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