Highly efficient immunoliposomes prepared with a method which is compatible with various lipid compositions.

@article{Holmberg1989HighlyEI,
  title={Highly efficient immunoliposomes prepared with a method which is compatible with various lipid compositions.},
  author={Ed K Holmberg and Kumiko Maruyama and David C Litzinger and Shannon B Wright and Michael A. Davis and George Walter Kabalka and Stephen J. Kennel and Li Huang},
  journal={Biochemical and biophysical research communications},
  year={1989},
  volume={165 3},
  pages={1272-8}
}
Monoclonal antibody was conjugated to N-glutaryl-phosphatidylethanolamine in the presence of octylglucoside by using N-hydroxysulfosuccinimide as a carboxyl-activation reagent. The conjugated antibody was then incorporated into liposomes by a simple dialysis method. The method is mild and is compatible with various lipid compositions of the liposomes. We have prepared immunoliposomes containing a lung endothelium-specific monoclonal antibody and showed excellent target binding (approximately 75… CONTINUE READING