Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases.

@article{Sung2014HighlyEG,
  title={Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases.},
  author={Young Hoon Sung and Jong Min Kim and Hyun-Taek Kim and Jaehoon Lee and Jisun Jeon and Young Tack Jin and Jung-Hwa Choi and Young Ho Ban and Sang-Jun Ha and Cheol-Hee Kim and Han-Woong Lee and Jin-Soo Kim},
  journal={Genome research},
  year={2014},
  volume={24 1},
  pages={
          125-31
        }
}
RNA-guided endonucleases (RGENs), derived from the prokaryotic Type II CRISPR-Cas system, enable targeted genome modification in cells and organisms. Here we describe the establishment of gene-knockout mice and zebrafish by the injection of RGENs as Cas9 protein:guide RNA complexes or Cas9 mRNA plus guide RNA into one-cell-stage embryos of both species. RGENs efficiently generated germline transmittable mutations in up to 93% of newborn mice with minimal toxicity. RGEN-induced mutations in the… CONTINUE READING

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