Highly efficient gene knockout by injection of TALEN mRNAs into oocytes and host transfer in Xenopus laevis

@inproceedings{Nakajima2015HighlyEG,
  title={Highly efficient gene knockout by injection of TALEN mRNAs into oocytes and host transfer in Xenopus laevis},
  author={Keisuke Nakajima and Yoshio Yaoita},
  booktitle={Biology open},
  year={2015}
}
Zinc-finger nucleases, transcription activator-like effector nucleases (TALENs) and the CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins) system are potentially powerful tools for producing tailor-made knockout animals. However, their mutagenic activity is not high enough to induce mutations at all loci of a target gene throughout an entire tadpole. In this study, we present a highly efficient method for introducing gene modifications at almost… CONTINUE READING

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Highly Influential
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