Highly efficient expression of homologous and heterologous genes in Bacillus megaterium


By developing an efficient transformation system it was possible to reintroduce two different glucose dehydrogenase genes of Bacillus megaterium into this host. These genes were previously cloned, sequenced and expressed in Escherichia coli. Since the expression of one of these genes (gdhA) turned out to be extremely high in B. megaterium, an expression… (More)
DOI: 10.1007/BF00296622


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