Highly efficient cell-mediated gene transfer using non-viral vectors and FuGene™6: in vitro and in vivo studies

  title={Highly efficient cell-mediated gene transfer using non-viral vectors and FuGene{\texttrademark}6: in vitro and in vivo studies
  author={Irina Hellgren and Viktor Drvota and Robert Pieper and Staffan Enoksson and Pontus Blomberg and Khalid Bin Islam and Christer Sylv{\'e}n},
  journal={Cellular and Molecular Life Sciences CMLS},
Abstract. The present study was undertaken to develop an efficient non-viral gene delivery system for cardiovascular gene therapy. We investigated transfection efficiency and toxic properties of the new transfection reagent, FuGene™6, and compared it with two other transfection reagents, Tfx™-50 and LipoTaxi™. For in vivo experiments, the plasmid was delivered intramuscularly via transplantation of fibroblasts transfected with plasmid and FuGene™6. Conditions for efficient gene delivery were… 


Three major approaches towards facilitating vector construction and improving vector expression cassette design are described and it is demonstrated that, heat shock at 42°C for 30 min, the pHi-Hot vector could achieve high gene expression levels while maintaining its inducibiiity.

Recent development of nonviral gene delivery systems with virus-like structures and mechanisms.

  • K. ItakaK. Kataoka
  • Biology
    European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
  • 2009

Novel amplifier expression vectors producing higher levels of IL-2 led to slower tumor growth and longer survival in vivo.

The results demonstrated that the transcriptional amplifier-based expression cassettes could be very useful in applications where high levels of gene expression are difficult to achieve.

Comparison of Transfection Efficiency of Nonviral Gene Transfer Reagents

Results suggest that FuGENE HD is the most preferred transfection reagent for many cell lines, followed by Arrest-In and jetPEI, and may be useful for improving nonviral gene and cell therapy applications.

Long-term efficient gene delivery using polyethylenimine with modified Tat peptide.

Recent developments in cationic lipid-mediated gene delivery and gene therapy

The very recent developments in the field of cationic lipids, both from industry and academia are reviewed, with designs adapted to recent findings in lipid-mediated transfection mechanisms.

Nucleofection: A New Method for Cutaneous Gene Transfer?

Compared with the established transfection reagent FuGENE6, transgene expression after nucleofection was earlier and higher than after lipofection, related to the method-dependent increase of cytotoxicity.

Effect of Vectors on Human Endothelial Cell Signal Transduction: Implications for Cardiovascular Gene Therapy

Investigation of the effect of viral and nonviral vectors on the phenotype and function of endothelial cells (ECs) and developed methods to block any activation caused by these vectors found that viral vectors, although efficient at transducing ECs, result in their activation.

Nucleofection Is an Efficient Nonviral Transfection Technique for Human Bone Marrow–Derived Mesenchymal Stem Cells

Nucleofection is an efficient nonviral transfection technique for human bone marrow– derived mesenchymal stem cells, which then may be used as cellular vehicles for the delivery of biological agents.

Effect of vectors on human endothelial cell signal transduction: implications for cardiovascular gene therapy.

  • P. TanS. Xue A. George
  • Biology, Medicine
    Arteriosclerosis, thrombosis, and vascular biology
  • 2006



High efficiency reporter gene transfection of vascular tissue in vitro and in vivo using a cationic lipid–DNA complex

The optimisation of gene transfer into vascular cells with this cationic lipid complex will be valuable for molecular studies of genes implicated in cardiovascular diseases and as a possible method of gene delivery with therapeutic intent.

Ionizing radiation greatly improves gene transfer efficiency in mammalian cells.

It is shown that ionizing radiation can improve plasmid transfection efficiency in both normal and neoplastic human and mouse cells, and the 2-hr delay described here, from the time of irradiation to the beginning of enhanced gene integration, suggests an inducible process that becomes active after the bulk of the radiation damage has been repaired.

Optimized galenics improve in vitro gene transfer with cationic molecules up to 1000-fold.

Gene transfer with a lipopolyamine (transfectam) in the presence of serum was increased over 10-fold by sequential addition of the lipid to DNA, and high complex concentrations led to large enhancements too, which points to the fact that formation of productive complexes is a slow process.

Increased gene expression after liposome-mediated arterial gene transfer associated with intimal smooth muscle cell proliferation. In vitro and in vivo findings in a rabbit model of vascular injury.

Findings indicate that the expression of liposome-mediated arterial gene transfer may be augmented in presence of ongoing cellular proliferation, as shown in rabbits with or without antecedent angioplasty.

Ultrasound-mediated transfection of mammalian cells.

Ultrasound-mediated transfection appears to be a promising method for gene transfer into mammalian cells using ultrasound transmitted through the walls of cell culture flasks or plates and was effective for suspended cells as well as for plated cells.

Efficient transfer of oligonucleotides and plasmid DNA into the whole heart through the coronary artery

Results clearly demonstrate that the hearts were efficiently transfected both by oligonucleotide and plasmid DNA as a result of coronary infusion of HVJ-liposome during cardioplegic arrest, providing a new tool for research and therapy for heart diseases.

Characterization of in vivo gene transfer into the arterial wall mediated by the Sendai virus (hemagglutinating virus of Japan) liposomes: an effective tool for the in vivo study of arterial diseases.

HVJ liposome-mediated arterial gene transfer is a highly efficient, noninvasive, and effective gene delivery method for the study of vascular disorders.

Site-specific gene expression in vivo by direct gene transfer into the arterial wall.

The results demonstrate that site-specific gene expression can be achieved by direct gene transfer in vivo and could be applied to the treatment of such human diseases as atherosclerosis or cancer.

On the mechanism of DNA transfection: efficient gene transfer without viruses

A transfection method for primary human fibroblasts that approaches the efficiency of viruses and the failure in HeLa cells of a mechanism for the elimination of foreign DNA implies strategies for optimizing gene transfer efficiency in virus-independent approaches to gene therapy.