Highly efficient cell-mediated gene transfer using non-viral vectors and FuGene™6: in vitro and in vivo studies

@article{Hellgren2000HighlyEC,
  title={Highly efficient cell-mediated gene transfer using non-viral vectors and FuGene™6: in vitro and in vivo studies
},
  author={I. Hellgren and V. Drvota and R. Pieper and S. Enoksson and P. Blomberg and K. Islam and C. Sylv{\'e}n},
  journal={Cellular and Molecular Life Sciences CMLS},
  year={2000},
  volume={57},
  pages={1326-1333}
}
  • I. Hellgren, V. Drvota, +4 authors C. Sylvén
  • Published 2000
  • Medicine, Biology
  • Cellular and Molecular Life Sciences CMLS
  • Abstract. The present study was undertaken to develop an efficient non-viral gene delivery system for cardiovascular gene therapy. We investigated transfection efficiency and toxic properties of the new transfection reagent, FuGene™6, and compared it with two other transfection reagents, Tfx™-50 and LipoTaxi™. For in vivo experiments, the plasmid was delivered intramuscularly via transplantation of fibroblasts transfected with plasmid and FuGene™6. Conditions for efficient gene delivery were… CONTINUE READING
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