Highly efficient DNA synthesis by the phage phi 29 DNA polymerase. Symmetrical mode of DNA replication.

  title={Highly efficient DNA synthesis by the phage phi 29 DNA polymerase. Symmetrical mode of DNA replication.},
  author={Luis Blanco and Ant{\'o}nio Bernad and Jose M. Lazaro and Guillaume Martin and Cristina Garmendia and M. Salas},
  journal={The Journal of biological chemistry},
  volume={264 15},
The results presented in this paper indicate that the phi 29 DNA polymerase is the only enzyme required for efficient synthesis of full length phi 29 DNA with the phi 29 terminal protein, the initiation primer, as the only additional protein requirement. Analysis of phi 29 DNA polymerase activity in various in vitro DNA replication systems indicates that two main reasons are responsible for the efficiency of this minimal system: 1) the phi 29 DNA polymerase is highly processive in the absence… CONTINUE READING
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Improvement of φ29 DNA polymerase amplification performance by fusion of DNA binding motifs.

Proceedings of the National Academy of Sciences of the United States of America • 2010
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