Highly effective in vivo gene transfection by blocked star vector.

Abstract

Nano-structured four branched, blocked star vector (BSV) was molecular designed by iniferter-based photo-living radical copolymerization method of N,N-dimethylaminopropylacrylamide and then N,N-dimethylacrylamide from four dithiocarbamate-derivatized benzene for a novel gene derivery non-viral vector. The conjugated pDNA complexes were satisfactorily stable in the condition of the electrophoresis. Under the charge ratio of 5/1 (vector/pDNA), complexes of BSV and pDNA (BSV-pDNA) demonstrated satisfied transfection efficiency into COS-1 cells with a little cytotoxicity. Mice injected with BSV-pDNA polyplexes showed a higher level of gene expression in either liver, kidney or spleen using the optimal charge ratio as determined in in vitro. These results suggest the potential use of BSV as a non-viral vector in clinic.

Cite this paper

@article{Zhou2005HighlyEI, title={Highly effective in vivo gene transfection by blocked star vector.}, author={Yanheng Zhou and Hui Ling Huang and Yosuke Nakayama}, journal={Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference}, year={2005}, volume={1}, pages={501-3} }