Highly effective cell synchronization in plant roots by hydroxyurea and amiprophos-methyl or colchicine.

@article{Pan1993HighlyEC,
  title={Highly effective cell synchronization in plant roots by hydroxyurea and amiprophos-methyl or colchicine.},
  author={W H Pan and Andreas Houben and R. Schlegel},
  journal={Genome},
  year={1993},
  volume={36 2},
  pages={387-90}
}
Effective somatic cell synchronization in root-tip meristems and improved chromosome spreading were achieved in white campion, wheat, rye, and barley by application of hydroxyurea and amiprophos-methyl or colchicine, combined with a pretreatment of ice water and modified fixative, as well as enzymatic digestion of the meristems. The protocol provides metaphase indices of approximately 50%. The chromosomes and chromosomal DNA were with minimum distortion, providing useful material for chromosome… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 32 extracted citations

Similar Papers

Loading similar papers…