Highly Simple and Sensitive Molecular Amplification-Integrated Fluorescence Anisotropy for Rapid and On-Site Identification of Adulterated Beef.

@article{Qiao2018HighlySA,
  title={Highly Simple and Sensitive Molecular Amplification-Integrated Fluorescence Anisotropy for Rapid and On-Site Identification of Adulterated Beef.},
  author={Dongqing Qiao and Jianguo Xu and Panzhu Qin and Li Yao and Jianfeng Lu and Sergei A. Eremin and Wei Chen},
  journal={Analytical chemistry},
  year={2018},
  volume={90 12},
  pages={
          7171-7175
        }
}
Fluorescence polarization (FP) signal is a self-referencing fluorescence signal, and it is less dependent on dye concentration and environmental interferences, which makes FP measurement a highly attractive alternative sensing technology to conventional fluorescent detection methods. Here we adopted a strategy for rapid increase of molecular weight to increase the FP signal for the detection of meat adulteration. The molecular weight of fluorescent labeled primers increased rapidly by slight… CONTINUE READING
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