Highly Sensitive Droplet Digital PCR Method for Detection of EGFR-Activating Mutations in Plasma Cell-Free DNA from Patients with Advanced Non-Small Cell Lung Cancer.

@article{Zhu2015HighlySD,
  title={Highly Sensitive Droplet Digital PCR Method for Detection of EGFR-Activating Mutations in Plasma Cell-Free DNA from Patients with Advanced Non-Small Cell Lung Cancer.},
  author={Guanshan Zhu and Xin Ye and Zhengwei Dong and Ya Chao Lu and Yun Sun and Yi Liu and Rose McCormack and Yi Gu and Xiaoqing Liu},
  journal={The Journal of molecular diagnostics : JMD},
  year={2015},
  volume={17 3},
  pages={265-72}
}
Epidermal growth factor receptor (EGFR) mutation testing in plasma cell-free DNA from lung cancer patients is an emerging clinical tool. However, compared with tissue testing, the sensitivity of plasma testing is not yet satisfactory because of the highly fragmented nature of plasma cell-free DNA, low fraction of tumor DNA, and limitations of available detection technologies. We therefore developed a highly sensitive and specific droplet digital PCR method for plasma EGFR mutation (exon19… CONTINUE READING