Highly Sensitive Assay for Dopamine‐β‐Hydroxylase Activity in Human Cerebrospinal Fluid by High Performance Liquid Chromatography‐Electrochemical Detection: Properties of the Enzyme

@article{Matsui1981HighlySA,
  title={Highly Sensitive Assay for Dopamine‐$\beta$‐Hydroxylase Activity in Human Cerebrospinal Fluid by High Performance Liquid Chromatography‐Electrochemical Detection: Properties of the Enzyme},
  author={Hiroaki Matsui and T. Kato and Chosaburo Yamamoto and Keisuke Fujita and Toshiharu Nagatsu},
  journal={Journal of Neurochemistry},
  year={1981},
  volume={37}
}
Abstract: This paper describes a new, sensitive assay for dopamine‐β‐hydroxylase (DBH) activity in human cerebrospinal fluid (CSF), serum and brain tissues by high performance liquid chromatography (HPLC) with electrochemical detection (ED). Dopamine (DA) was used as a substrate and was incubated under optimal conditions. Norepinephrine (NE) formed enzymatically from DA was isolated by a double‐column procedure, the first column of Dowex‐50‐H+ and the second column of aluminum oxide. NE was… 

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