Highly Sensitive Assay for Dopamine‐β‐Hydroxylase Activity in Human Cerebrospinal Fluid by High Performance Liquid Chromatography‐Electrochemical Detection: Properties of the Enzyme

  title={Highly Sensitive Assay for Dopamine‐$\beta$‐Hydroxylase Activity in Human Cerebrospinal Fluid by High Performance Liquid Chromatography‐Electrochemical Detection: Properties of the Enzyme},
  author={Hiroaki Matsui and T. Kato and Chosaburo Yamamoto and Keisuke Fujita and Toshiharu Nagatsu},
  journal={Journal of Neurochemistry},
Abstract: This paper describes a new, sensitive assay for dopamine‐β‐hydroxylase (DBH) activity in human cerebrospinal fluid (CSF), serum and brain tissues by high performance liquid chromatography (HPLC) with electrochemical detection (ED). Dopamine (DA) was used as a substrate and was incubated under optimal conditions. Norepinephrine (NE) formed enzymatically from DA was isolated by a double‐column procedure, the first column of Dowex‐50‐H+ and the second column of aluminum oxide. NE was… 

Determination of Dopamine-β-hydroxylase Activity in Human Serum Using UHPLC-PDA Detection

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A highly sensitive new fluorometric assay for DBH activity by high-performance liquid chromatography is devised and applied to the assay ofDBH activity in human cerebrospinal fluid.

Photometric assay of dopamine- -hydroxylase activity in human blood.

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Among the patients with bipolar affective disorder, DBH activity from manic patients was significantly lower than that from depressed or euthymic patients, and the theory that the amount of DBH in CSF may serve as an indicator of central noradrenergic activity is discussed.