Highly Precise Measurement of HIV DNA by Droplet Digital PCR

  title={Highly Precise Measurement of HIV DNA by Droplet Digital PCR},
  author={Matthew C. Strain and Steven M. Lada and Tiffany Luong and Steffney E. Rought and Sara Gianella and Valeri Terry and Celsa A Spina and Christopher H. Woelk and Douglas D. Richman},
  booktitle={PloS one},
Deoxyribonucleic acid (DNA) of the human immunodeficiency virus (HIV) provides the most sensitive measurement of residual infection in patients on effective combination antiretroviral therapy (cART). Droplet digital PCR (ddPCR) has recently been shown to provide highly accurate quantification of DNA copy number, but its application to quantification of HIV DNA, or other equally rare targets, has not been reported. This paper demonstrates and analyzes the application of ddPCR to measure the… CONTINUE READING
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