Higher-plant cofactor-independent phosphoglyceromutase: purification, molecular characterization and expression

  title={Higher-plant cofactor-independent phosphoglyceromutase: purification, molecular characterization and expression},
  author={Yafan Huang and Stephen D. Plakeley and Sybil McAleese and Linda A Fothergill-Gilmore and David T. Dennis},
  journal={Plant Molecular Biology},
Cofactor-independent phosphoglyceromutase (PGM) was purified to homogeneity from developing castor seed endosperm. Immunological characterization using monospecific antisera raised against this protein indicates that the enzyme is located in the cytosol and that there is no immunologically related polypeptide in the leucoplast from this tissue. Isolation and sequence determination of full-length cDNA clones for castor and tobacco PGM demonstrate that the protein is highly conserved in these… CONTINUE READING


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