This paper investigates the use of isothermal titration calorimetry (ITC) as a tool for studying molecular systems in which weaker secondary interactions are present in addition to a dominant primary interaction. Such systems are challenging since the signal pertaining to the stronger primary interaction tends to overshadow the signal from the secondary… (More)

Figure 5. Temperature dependence of binding enthalpies (ΔH) for the primary (A) and the secondary sites (B), obtained by global fits of the 1 and 2 site model to 3 titrations at each temperature. Enthalpies obtained with the 2 site model are represented by closed symbols (GDC: ■, GC: ●) while the 1 site model yields the open symbols (GDC: □, GC: ○). The error bars represent the 95% asymptotic confidence intervals. Values of ΔCp are calculated as the slope of the lines and listed in the text boxes along with the 95% confidence intervals. The plotted data are from Table 1.