Higher catalytic efficiency of N-7-methylation is responsible for processive N-7 and 2'-O methyltransferase activity in dengue virus.

@article{Chung2010HigherCE,
  title={Higher catalytic efficiency of N-7-methylation is responsible for processive N-7 and 2'-O methyltransferase activity in dengue virus.},
  author={Ka Yan Chung and Hongping Dong and Alexander Theodore Chao and Pei-Yong Shi and Julien Lescar and Siew Pheng Lim},
  journal={Virology},
  year={2010},
  volume={402 1},
  pages={
          52-60
        }
}
Methyltransferases (MTases) from the genus Flavivirus encode both N-7 and 2'-O activities needed for type 1 (m(7)GpppNm) cap structure formation. We performed kinetic studies to understand the mechanisms of its progressive N-7 and 2'-O methylations. Sequential N-7 to 2'-O methylation occurred via a random bi bi and processive mechanism that does not involve enzyme-RNA dissociation. Analyses of steady state kinetic parameters showed that N-7 precedes 2'-O methylation as it turnovers RNA faster… CONTINUE READING
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