High yields of monomeric recombinant beta-interferon from macroporous microcarrier cultures under hypothermic conditions.

@article{Tharmalingam2008HighYO,
  title={High yields of monomeric recombinant beta-interferon from macroporous microcarrier cultures under hypothermic conditions.},
  author={Tharmala Tharmalingam and Kevin Sunley and Michael Butler},
  journal={Biotechnology progress},
  year={2008},
  volume={24 4},
  pages={832-8}
}
Macroporous microcarriers such as Cytopore entrap mammalian cells in a mesh network allowing growth to high cell concentrations in a protected environment within a stirred culture. Chinese hamster ovary (CHO) cells producing recombinant human beta-interferon (IFN-beta) and grown in Cytopore microcarriers showed a 2.6- to 2.8-fold increase in the volumetric product titer compared with cells grown in an equivalent suspension culture. In an attempt to maximize production of IFN-beta, microcarrier… CONTINUE READING

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