High-yielding enzymatic method for isolation and culture of microvascular endothelial cells from bovine retinal blood vessels.

@article{Banumathi2009HighyieldingEM,
  title={High-yielding enzymatic method for isolation and culture of microvascular endothelial cells from bovine retinal blood vessels.},
  author={Elayappan Banumathi and Ravinarayanan Haribalaganesh and S Sheik Pran Babu and Nirbhay Kumar and Gurunathan Sangiliyandi},
  journal={Microvascular research},
  year={2009},
  volume={77 3},
  pages={377-81}
}
A simple and non-mechanical enzymatic method for the isolation and large-scale in vitro culture of bovine retinal endothelial cells (BRECs) is described. A sufficiently high yield of retinal microvessels was obtained from bovine eyes by brisk stirring of the retina with Minimum Essential Medium for 3 min, washing the pellet five times by repeated centrifugation at 300 x g and sieving in a single step. Pericyte contamination was eliminated by increasing the incubation period in an enzyme mixture… CONTINUE READING

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