High-yield spore production from Bacillus licheniformis by solid state fermentation

@article{Zhao2007HighyieldSP,
  title={High-yield spore production from Bacillus licheniformis by solid state fermentation},
  author={Shumiao Zhao and Nan Hu and Jun Huang and Yunxiang Liang and Bin Zhao},
  journal={Biotechnology Letters},
  year={2007},
  volume={30},
  pages={295-297}
}
Bacillus licheniformis was grown for 48 h at 37°C in solid state fermentation; a maximum of 1.7 × 1011 spores/g dry substrate were obtained using rice straw powder (300 g/kg) and wheat bran (700 g/kg) supplemented with glucose (40 g/kg), peptone (20 g/kg), yeast extract (20 g/kg), KH2PO4 (10 g/kg) and CaO (5 g/kg) with an initial moisture content of 65%. 

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