High-yield recovery of electroblotted proteins and cleavage fragments from a cationic polyvinylidene fluoride-based membrane.

@article{Patterson1992HighyieldRO,
  title={High-yield recovery of electroblotted proteins and cleavage fragments from a cationic polyvinylidene fluoride-based membrane.},
  author={Scott Patterson and Daniel R Hess and T Yungwirth and Ruedi Aebersold},
  journal={Analytical biochemistry},
  year={1992},
  volume={202 1},
  pages={193-203}
}
In this report we describe the use of a novel, experimental, polyvinylidene fluoride-based membrane with a cationic surface for the isolation by electroblotting of small amounts of proteins separated by gel electrophoresis for further characterization by protein fragmentation for internal sequence analysis. The membrane is characterized by a surface that… CONTINUE READING