High-yield membrane protein expression from E. coli using an engineered outer membrane protein F fusion.

@article{Su2013HighyieldMP,
  title={High-yield membrane protein expression from E. coli using an engineered outer membrane protein F fusion.},
  author={Pin-Chuan Su and William W. Si and Deidre L Baker and Bryan W Berger},
  journal={Protein science : a publication of the Protein Society},
  year={2013},
  volume={22 4},
  pages={434-43}
}
Obtaining high yields of membrane proteins necessary to perform detailed structural study is difficult due to poor solubility and variability in yields from heterologous expression systems. To address this issue, an Escherichia coli-based membrane protein overexpression system utilizing an engineered bacterial outer membrane protein F (pOmpF) fusion has been developed. Full-length human receptor activity-modifying protein 1 (RAMP1) was expressed using pOmpF, solubilized in FC15 and purified to… CONTINUE READING