High-yield expression and purification of a monotopic membrane glycosyltransferase.


Membrane proteins are essential to many cellular processes. However, the systematic study of membrane protein structure has been hindered by the difficulty in obtaining large quantities of these proteins. Protein overexpression using Escherichia coli is commonly used to produce large quantities of protein, but usually yields very little membrane protein… (More)
DOI: 10.1016/j.pep.2009.03.008


4 Figures and Tables