High yield cell-free production of integral membrane proteins without refolding or detergents.

Abstract

Integral membrane proteins act as critical cellular components and are important drug targets. However, difficulties in producing membrane proteins have hampered investigations of structure and function. In vivo production systems are often limited by cell toxicity, and previous in vitro approaches have required unnatural folding pathways using detergents… (More)
DOI: 10.1016/j.bbamem.2008.01.023

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