High-voltage electroporation of bacteria: genetic transformation of Campylobacter jejuni with plasmid DNA.

@article{Miller1988HighvoltageEO,
  title={High-voltage electroporation of bacteria: genetic transformation of Campylobacter jejuni with plasmid DNA.},
  author={Jeff Miller and W. J. Dower and Lucy S. Tompkins},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1988},
  volume={85 3},
  pages={856-60}
}
Electroporation permits the uptake of DNA by mammalian cells and plant protoplasts because it induces transient permeability of the cell membrane. We investigated the utility of high-voltage electroporation as a method for genetic transformation of intact bacterial cells by using the enteric pathogen Campylobacter jejuni as a model system. This report demonstrates that the application of high-voltage discharges to bacterial cells permits genetic transformation. Our method involves exposure of a… CONTINUE READING
Recent Discussions
This paper has been referenced on Twitter 5 times over the past 90 days. VIEW TWEETS
49 Citations
1 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 49 extracted citations

References

Publications referenced by this paper.

Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY)

  • N. Engl
  • J. Med
  • 1983

Similar Papers

Loading similar papers…