High tolerance against Chrysomela tremulae of transgenic poplar plants expressing a synthetic cry3Aa gene from Bacillus thuringiensis ssp tenebrionis

@article{Gnissel2004HighTA,
  title={High tolerance against Chrysomela tremulae of transgenic poplar plants expressing a synthetic cry3Aa gene from Bacillus thuringiensis ssp tenebrionis},
  author={Anne G{\'e}nissel and Jean-Charles Lepl{\'e} and Nad{\`e}ge Millet and Sylvie Augustin and Lise Jouanin and Gilles Pilate},
  journal={Molecular Breeding},
  year={2004},
  volume={11},
  pages={103-110}
}
Hybrid poplars (Populus tremula ×Populus tremuloides) have been genetically engineered viaAgrobacterium tumefaciens, to express a syntheticcry3Aa gene derived from the native Bacillusthuringiensis subsp. tenebrionis cry3Aa gene.The presence and the expression of the transgene have been verified in fourtransgenic poplar lines, using Southern, northern and western analyses. Thetransgenic poplar's toxicity towards the phytophagous beetleChrysomela tremulae (Coleoptera, Chrysomelidae) has… CONTINUE READING
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