High throughput virus-induced gene silencing implicates heat shock protein 90 in plant disease resistance.

@article{Lu2003HighTV,
  title={High throughput virus-induced gene silencing implicates heat shock protein 90 in plant disease resistance.},
  author={Rui Lu and Isabelle Malcuit and Peter Moffett and Mar{\'i}a Teresa Castellanos Ruiz and Jack R. Peart and Ai-Jiuan Wu and John Paul Rathjen and Abdelhafid Bendahmane and Louise Day and David C. Baulcombe},
  journal={The EMBO journal},
  year={2003},
  volume={22 21},
  pages={5690-9}
}
Virus-induced gene silencing was used to assess the function of random Nicotiana benthamiana cDNAs in disease resistance. Out of 4992 cDNAs tested from a normalized library, there were 79 that suppressed a hypersensitive response (HR) associated with Pto-mediated resistance against Pseudomonas syringae. However, only six of these clones blocked the Pto-mediated suppression of P.syringae growth. The three clones giving the strongest loss of Pto resistance had inserts corresponding to HSP90 and… CONTINUE READING
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