High-throughput tissue extraction protocol for NMR- and MS-based metabolomics.

  title={High-throughput tissue extraction protocol for NMR- and MS-based metabolomics.},
  author={Huifeng Wu and Andrew D Southam and Adam Hines and Mark R. Viant},
  journal={Analytical biochemistry},
  volume={372 2},
In metabolomics, tissues typically are extracted by grinding in liquid nitrogen followed by the stepwise addition of solvents. This is time-consuming and difficult to automate, and the multiple steps can introduce variability. Here we optimize tissue extraction methods compatible with high-throughput, reproducible nuclear magnetic resonance (NMR) spectroscopy- and mass spectrometry (MS)-based metabolomics. Previously, we concluded that methanol/chloroform/water extraction is preferable for… CONTINUE READING
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