High-throughput stem-loop RT-qPCR miRNA expression profiling using minute amounts of input RNA

@inproceedings{Mestdagh2008HighthroughputSR,
  title={High-throughput stem-loop RT-qPCR miRNA expression profiling using minute amounts of input RNA},
  author={Pieter Mestdagh and Tom Feys and Nathalie Bernard and Simone M. Guenther and Caifu Chen and Frank Speleman and Jo Vandesompele},
  booktitle={Nucleic acids research},
  year={2008}
}
MicroRNAs (miRNAs) are an emerging class of small non-coding RNAs implicated in a wide variety of cellular processes. Research in this field is accelerating, and the growing number of miRNAs emphasizes the need for high-throughput and sensitive detection methods. Here we present the successful evaluation of the Megaplex reverse transcription format of the stem-loop primer-based real-time quantitative polymerase chain reaction (RT-qPCR) approach to quantify miRNA expression. The Megaplex… CONTINUE READING